Operations Manual

Assembly
1) Screw the fiber optic adjustment screw to the bottom plate with unthreaded portion pointing to the outside of the cultivator (approximately half way threw).
2) Screw the top of the fiber optic adjustment screw to the fiber optic adjustment screw with larger diameter of the top facing up (use super glue or silicon tape to secure the top of the fiber optic adjustment screw to the fiber optic adjustment screw).
3) Slide one end of the fiber optic cable threw the adjustment screw.
4) Screw the end of the fiber optic cable, which exits the top of the adjustment screw, to the connection on the fiber optic slider.
5) Slide the fiber optic slider threw the three G-10 struts with fiber optic cable facing down.
6) Make sure the top of the adjustment screw seats flush with the fiber optic slider.
7) Align the three holes of the fiber optic support top with the three G-10 struts and press together (it should be a tight fit so press hard).
8) Swing the fiber optic safety arm into place and tighten the screw (this prevents the adjustment screw from separating from the fiber optic slider).
9) Position the water jacket so the bottom faces up (this is when the two 1/2 inch threaded holes are facing down)
10) Apply gasket sealant to the water jacket surface (use sealant sparingly and DO NOT allow the sealant to enter holes).
11) Place the bottom gasket on the bottom of the water jacket (align the holes).
12) Apply gasket sealant to the bottom gasket surface (use sealant sparingly).
13) Insert the fiber optic support assembly into the water jacket (align the holes of the bottom with the water jacket and gasket holes) and press together.
14) Screw in all five 1/4 inch -20 stainless steel bolts until snug (DO NOT OVER TIGHTEN).
15) Wipe excess sealant from water jacket.
16) Turn the entire unit upside down and slide it on the stand (fiber optic adjustment screw slides threw the slot in stand).
17) Insert two aluminum (5/32 inch diameter-2.25 inch length) tubes into cuvett stopper (allow 3/4 inches to hang from the bottom (small end) of the cuvett stopper).
18) Insert one stainless steel (1/4 inch diameter-5.375 inch length) tube into cuvett stopper (allow 3.5 inches to hang from the bottom (small end) of the cuvett stopper).
19) Attach two surgical tubes one to one of the aluminum (5/32 inch diameter-2.25 inch length) tubes and the other to the stainless steel (1/4 inch diameter-5.375 inch length) tube. The end of the surgical tubes should be attached to the ends of the tubes coming out of the top of the cuvett stopper (set aside).
20) Insert the 1/2 inch stainless steel tubing into the top of the water jacket stopper (allow 4.375 inches to hang from the bottom of this stopper and have the bent end of the tube at the top of the stopper).
21) Insert the two aluminum (5/32 inch diameter-2.75 inch length) and one aluminum (1/4 inch diameter-2.75 inch length) tubes into the three holes in the water jacket stopper (allow 1.125 inches of these tubes to hang out from the bottom of this stopper).
22) Slide the top plate up and press the water jacket stopper threw the plate until the water jacket stopper's lip rests on the top plate (make sure the flat sides of the water jacket stopper are aligned with the two inside holes on the top plate).
23) Insert the end of the 1/2 inch stainless steel tube, coming out of the bottom of the water jacket stopper, into the top of the cuvett stopper (allow 1.25 inches of the 1/2 inch stainless steel tube to poke out of the bottom of the cuvett stopper).
24) Insert the thermocouple wire into the aluminum tube coming out of the water jacket stopper. Slide the wire threw this tube and threw the tube coming out of the cuvett stopper use gasket sealant to plug up the tube exiting the cultivator (allow enough wire to hang out of the tube, so the wire is located in the yeast medium).
25) Attach the two surgical hoses to the aluminum 5/32 inch tube and aluminum 1/4 inch tube.
26) Use silicon to seal the water jacket stopper to the top plate (silicon both the bottom and top).
27) Check the distances in steps 15,16,18,19 and 20.
28) Align the holes on the bottom of the cultivator support assembly with the two inside holes on the top plate.
29) Screw the cultivator support assembly to the top plate using two stainless steel 1/4 inch-20 screws (DO NOT OVER TIGHTEN).
30) Apply gasket sealant to the water jacket surface (use sealant sparingly and DO NOT allow the sealant to enter holes).
31) Place the top gasket on the water jacket top (align all of the holes).
32) Apply gasket sealant to the top gasket surface (use sealant sparingly).
33) Insert the cuvett stopper into the cuvett (press tightly into place).
34) While holding the cuvett into place, insert cuvett into top of water jacket and threw the hole of the fiber optic support top (press down on the top plate).
35) Screw in all five 1/4 inch-28 stainless steel bolts half way down.
36) Wipe excess sealant from water jacket.
37) Screw in the two inlet and outlet tubes (apply silicon tape to their threads for a water tight seal).
38) Screw down the five screws on the top until snug (DO NOT OVER TIGHTEN).
39) Attach the clear 1/4 inch tubing to one end of the sampling insertion rod and 1/4 inch surgical tubing to the other.
40) Slide the small rubber stopper on to the sampling insertion rod (apply Vaseline to the sampling insertion rod to allow for smoother sliding).
41) Attach the syringe to the free end of the surgical tubing.
42) Attach the sampling tip to the free end of the clear tubing.

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Setup
1) Attach the two clear 1/2 inch hoses to both the inlet and outlet tubes, located on top of the water jacket (use three zip ties per connection to hold in place).
2) Attach the CO2 hose to the aluminum CO2 tube (located on top of the cultivator).
3) Attach the oxygen hose to the stainless steel tube (located on top of the cultivator).
4) Attach the cultivator insertion rod to the cultivator assembly mount by screwing the rod in.
5) Attach the insertion rod support to the insertion rod and insert pin.
6) Insert the sampling insertion rod into the PVC tube with the syringe exiting the end of the PVC with out the connector for the sampling tube (slide the tube threw the stopper so that the clear tubing pokes out the other end).
7) Attach the connector end of the PVC pipe to the 1/2 inch stainless steel tube (make sure that the clear tube poking out slides smoothly into the sampling tube).
8) Insert the free end of the fiber optics threw the bore of the magnet.
9) Slowly lower the cultivator into the bore of the magnet with the insertion rod and rest the insertion rod support on the outer diameter of the magnet bore.
10) Connect the fiber optics to the spectrometer.
11) Connect the oxygen tube to the oxygen tank.
12) Connect the inlet hose to the outlet of the pump.
13) Insert the pump into a bucket full of water.
14) Insert the outlet hose into the bucket full of water.
15) Insert the heating element into the bucket of water.
16) Insert one of the thermocouple wires into the bucket of water.
17) Connect the thermocouple wires to its display unit.

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Operation:
1) Turn on the heating element and allow the bucket of water to reach a steady state temperature of 30 deg.
2) Turn on the magnet
3) Turn on the pump
4) Turn on the spectrometer
5) Turn on the thermocouple display unit
6) To take samples simply insert the sampling insertion rod to the yeast medium and take desired sample with syringe.
7) Slide the insertion rod threw the stopper until the clear tube has cleared the PVC valve and shut the valve off.
8) Then pop the stopper off the PVC pipe and take the entire sampling insertion rod out of the PVC pipe.
9) Reverse steps 8-6 to take another sample.

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Last Revised: 2000-12-20